​​Clinical Neuropathology & Diagnostic Biomarkers

Molecular & Cellular Neurodegeneration

​​​Digital Pathology & AI

Selective immunolabeling of tau aggregates containing tau with four microtubule-binding domain repeats (4R), but not three (3R), in post-mortem human tauopathy brains with antisera targeting JADE1 protein. Immunohistochemical staining with phospho-tau (p-tau) specific antisera (AT8) and JADE1 specific antisera demonstrates neurofibrillary tangle (NFT) formation marked by the presence of JADE1 in specific populations of neurons and glia.

(a, b) Primary age-related tauopathy (PART, n = 3) NFTs contain JADE1 positive staining in the soma and neurites in the entorhinal cortex.

(c, d) Alzheimer disease (AD, n = 3) individual with Aβ and AT8-positive neuritic plaques and NFTs (subiculum) also display JADE1 immunopositivity in dystrophic neurites and NFTs.

(e, f) Chronic traumatic encephalopathy (CTE, n = 3) contains positive p-tau staining around a blood vessel in the depth of a neocortical sulcus that is immunopositive for JADE1.